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embryonic mouse fibroblast cell line nih3t3  (ATCC)


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    ATCC embryonic mouse fibroblast cell line nih3t3
    Embryonic Mouse Fibroblast Cell Line Nih3t3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 13300 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+embryonic+fibroblast+nih+3t3+cell+line/NIH%2F3T3/10__1007_slash_s12195___026___00945___7-81-0-9
    Average 99 stars, based on 13300 article reviews
    embryonic mouse fibroblast cell line nih3t3 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Generated:

    Article Title: Bispecific antigen binding molecules targeting OX40 and FAP
    Article Snippet: .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection.

    Article Title: Bispecific 2+1 contorsbodies
    Article Snippet: .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. ..

    Article Title: Bispecific antigen binding molecules capable of specific binding to CD40 and to fap
    Article Snippet: Purification of the Bispecific Antibodies Antibodies were purified from cell culture supernatants by affinity chromatography using MabSelect Sure-SEPHAROSE (GE Healthcare, Sweden) chromatography. .. NIH/3T3-huFAP clone 19 and NIH/3T3-mFAP clone 26 were generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express under 1.5 μg/mL Puromycin selection huFAP or mFAP, respectively. .. After neutralization with 1 M Tris pH 9.0, aggregated protein was separated from monomeric antibody species by size exclusion chromatography (SUPERDEX 200, GE Healthcare) in 20 mM histidine, 140 mM NaCl, pH 6.0.

    Transfection:

    Article Title: Bispecific antigen binding molecules targeting OX40 and FAP
    Article Snippet: .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection.

    Article Title: Bispecific 2+1 contorsbodies
    Article Snippet: .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. ..

    Article Title: Bispecific antigen binding molecules capable of specific binding to CD40 and to fap
    Article Snippet: Purification of the Bispecific Antibodies Antibodies were purified from cell culture supernatants by affinity chromatography using MabSelect Sure-SEPHAROSE (GE Healthcare, Sweden) chromatography. .. NIH/3T3-huFAP clone 19 and NIH/3T3-mFAP clone 26 were generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express under 1.5 μg/mL Puromycin selection huFAP or mFAP, respectively. .. After neutralization with 1 M Tris pH 9.0, aggregated protein was separated from monomeric antibody species by size exclusion chromatography (SUPERDEX 200, GE Healthcare) in 20 mM histidine, 140 mM NaCl, pH 6.0.

    Expressing:

    Article Title: Bispecific antigen binding molecules targeting OX40 and FAP
    Article Snippet: .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection.

    Article Title: Bispecific 2+1 contorsbodies
    Article Snippet: .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. ..

    Article Title: Bispecific antigen binding molecules capable of specific binding to CD40 and to fap
    Article Snippet: Purification of the Bispecific Antibodies Antibodies were purified from cell culture supernatants by affinity chromatography using MabSelect Sure-SEPHAROSE (GE Healthcare, Sweden) chromatography. .. NIH/3T3-huFAP clone 19 and NIH/3T3-mFAP clone 26 were generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express under 1.5 μg/mL Puromycin selection huFAP or mFAP, respectively. .. After neutralization with 1 M Tris pH 9.0, aggregated protein was separated from monomeric antibody species by size exclusion chromatography (SUPERDEX 200, GE Healthcare) in 20 mM histidine, 140 mM NaCl, pH 6.0.

    Selection:

    Article Title: Bispecific antigen binding molecules targeting OX40 and FAP
    Article Snippet: .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection.

    Article Title: Bispecific 2+1 contorsbodies
    Article Snippet: .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. ..

    Article Title: Bispecific antigen binding molecules capable of specific binding to CD40 and to fap
    Article Snippet: Purification of the Bispecific Antibodies Antibodies were purified from cell culture supernatants by affinity chromatography using MabSelect Sure-SEPHAROSE (GE Healthcare, Sweden) chromatography. .. NIH/3T3-huFAP clone 19 and NIH/3T3-mFAP clone 26 were generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express under 1.5 μg/mL Puromycin selection huFAP or mFAP, respectively. .. After neutralization with 1 M Tris pH 9.0, aggregated protein was separated from monomeric antibody species by size exclusion chromatography (SUPERDEX 200, GE Healthcare) in 20 mM histidine, 140 mM NaCl, pH 6.0.

    other:

    Article Title: Tumor-targeted agonistic CD28 antigen binding molecules
    Article Snippet: TABLE 1 Summary of the production and purification of bispecific or trispecific CD28 antigen binding molecules Analytical Purity SEC (HMW/ measured Yield Monomer/ by CE- Molecule Description [mg/l] LMW) [%] SDS [%] A CD28(SA) 257 0/100/0 84.25 (hu IgG4) B CD28(SA) 390 0/97.3/2.7 84 hu IgG1 (PG-LALA) C FAP(4B9) − 19.5 0.64/97.28/2.07 98.75 CD28(SA) 1 + 1 D FAP(4B9) − 1.75 3.53/96.48/0 n.d. CD28(TGN1412) 1 + 4 E FAP(4B9) − 0.38 0.8/95.48/3.72 93.58 CD28(SA) 1 + 2 F FAP(4B9) − 18.2 1.4/98.6/0 91.42 CD28(SA) 2 + 2 G FAP (4B9) − 2.66 3.79/94.02/2.19 64 CD28 (SA) 2 + 1 H FAP(4B9) − 10.6 0/100/0 99.38 CD28(SA) C-01 I FAP(4B9) − 5.55 4.12/81.17/14.71 96.5 CD28(SA) C-04 J CEA − 6.25 1/99/0 n.d. CD28(SA) 2 + 2 K CEA − 5.8 0.5/99.5/0 64 CD28(SA) 1 + 2 L monovalent IgG1 38.5 0.2/99.6/0.2 99.3 CD28 (SA) M CEA − 14.3 0/100/0 99.18 CD28(SA) 1 + 1 N CD28 (mAb 9.3) 22.06 0/100/0 88 hu IgG1 (PG-LALA) O FAP(4B9) − 2.14 0/100/0 97.4 CD28(mAb9.3) C-03 P FAP(4B9) − 7.6 1.2/98.8/0 97.6 CD28(mAb9.3) 1 + 4 Q FAP(4B9) − 16.

    Article Title: Combination therapy with targeted 4-1BB (CD137) agonists/anti-FAP binding domain and anti-CEA/anti-CD3 bispecific antibody
    Article Snippet: Ten million of isolated CD8 T cells were resuspended in 1 mL sterile DPBS supplemented with 1% (v/v) FBS along with 50 μL of PE-labeled HLA-A2-pentamer containing the CMV-derived NLVPMVATV peptide (SEQ ID NO: 169) (ProImmune, Cat. No. F008-2B) and incubated for 10 min at room temperature.

    Article Title: Methods of treating cancer by administering tumor necrosis factor (TNF) family ligand trimer-containing antigen-binding molecules
    Article Snippet: The surface expression of FAP was quantified using the Quifikit (Dako Cat. No. K0078) according to manufactures instructions.

    Article Title: Bispecific antibodies specific for OX40
    Article Snippet: 0.5×105 NIH/3T3-huFAP clone 39 or WM266-4 cells were then added to each well of a round-bottom suspension cell 96-well plates (greiner bio-one, cellstar, Cat. No. 650185) and the binding assay was performed in a similar manner as described in Example 2.3.2.

    Plasmid Preparation:

    Article Title: Bispecific antigen binding molecules targeting OX40 and FAP
    Article Snippet: .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. ..



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    Representative image of the subcutaneous implantation site of <t>the</t> <t>NIH/3T3</t> group. H&E shows a large subcutaneous mass and ink in the middle. The upper left corner shows a detail of the ink-bearing cells. The picture was taken at 10×; 80 µm. Close-up picture (upper left corner) was taken at 40×; 20 µm.
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    Representative image of the subcutaneous implantation site of the NIH/3T3 group. H&E shows a large subcutaneous mass and ink in the middle. The upper left corner shows a detail of the ink-bearing cells. The picture was taken at 10×; 80 µm. Close-up picture (upper left corner) was taken at 40×; 20 µm.

    Journal: Cells

    Article Title: Preclinical Tumorigenicity Study of an Advanced Therapy Medicinal Product for Diffuse Cartilage Lesions in an Osteoarthritic Environment

    doi: 10.3390/cells15050429

    Figure Lengend Snippet: Representative image of the subcutaneous implantation site of the NIH/3T3 group. H&E shows a large subcutaneous mass and ink in the middle. The upper left corner shows a detail of the ink-bearing cells. The picture was taken at 10×; 80 µm. Close-up picture (upper left corner) was taken at 40×; 20 µm.

    Article Snippet: The mouse embryonic fibroblast cell line NIH/3T3 (ATCC; LGC Standards GmbH, Wesel, Germany) was used as a negative control in this study.

    Techniques: