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human cell lines nih 3t3 mouse embryonic fibroblasts  (ATCC)


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    ATCC human cell lines nih 3t3 mouse embryonic fibroblasts
    Human Cell Lines Nih 3t3 Mouse Embryonic Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 13229 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+embryonic+fibroblast+nih+3t3+cell+line/NIH%2F3T3/pm42229414-756-2-9
    Average 99 stars, based on 13229 article reviews
    human cell lines nih 3t3 mouse embryonic fibroblasts - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Generated:

    Article Title: Bispecific antigen binding molecules targeting OX40 and FAP
    Article Snippet: .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. .. The lack of binding to OX40 negative FAP negative tumor cells was tested using A549 NucLightTM Red Cells (Essen Bioscience, Cat. No. 4491) expressing the NucLight Red fluorescent protein restricted to the nucleus to allow separation from unlabeled human FAP positive NIH/3T3-huFAP clone 19 cells.

    Article Title: Bispecific 2+1 contorsbodies
    Article Snippet: .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. ..

    Article Title: Tumor-targeted agonistic CD28 antigen binding molecules
    Article Snippet: .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. .. The binding to human CD28 was tested with CHO cells expressing human CD28 (parental cell line CHO-kl ATCC #CCL-61, modified to stably overexpress human CD28).

    Article Title: Combination therapy with targeted 4-1BB (CD137) agonists/anti-FAP binding domain and anti-CEA/anti-CD3 bispecific antibody
    Article Snippet: NLV-peptide-specific CD8+ T cells were isolated by FACS sorting using an ARIA cell sorter (BD Bioscience with DIVA software) with the following settings: 100 μm nozzle and purity sort mask. .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP. .. Cells were cultured in DMEM (GIBCO, Cat. No. 42430-082) containing 10% calf serum (Sigma-Aldrich, Cat. No. C8056-500 ml, gamma-irradiated, mycoplasma-free and heat inactivated at 56° C. for 35 min) and 1.5 μg/mL Puromycin (Sigma-Aldrich, Cat. No. ant-pr-1). b) Preparation of Effector cells Buffy coats were obtained from the Zürich blood donation center.

    Article Title: Bispecific antigen binding molecules capable of specific binding to CD40 and to fap
    Article Snippet: .. NIH/3T3-huFAP clone 19 and NIH/3T3-mFAP clone 26 were generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express under 1.5 μg/mL Puromycin selection huFAP or mFAP, respectively. ..

    Article Title: Methods of treating cancer by administering tumor necrosis factor (TNF) family ligand trimer-containing antigen-binding molecules
    Article Snippet: .. NIH/3T3-huFAP clone 39 was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. .. The surface expression of FAP was quantified using the Quifikit (Dako Cat. No. K0078) according to manufactures instructions.

    Article Title: Bispecific antibodies specific for OX40
    Article Snippet: 4.3.2.2 Binding to Human FAP-Expressing Tumor Cells The binding to cell surface FAP was tested using human fibroblast activating protein (huFAP) expressing cells NIH/3T3-huFAP clone 39 or WM266-4 cells (ATCC CRL-1676). .. NIH/3T3-huFAP clone 39 was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. .. In some assays WM266-4 cells were pre-labeled with PKH-26 Red Fluorescence Cell linker Kit (Sigma, Cat.-No. PKH26GL) as described in Example 2.3.2 to allow separation of these tumor cells from other cells present (e.g. human PBMC).

    Transfection:

    Article Title: Bispecific antigen binding molecules targeting OX40 and FAP
    Article Snippet: .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. .. The lack of binding to OX40 negative FAP negative tumor cells was tested using A549 NucLightTM Red Cells (Essen Bioscience, Cat. No. 4491) expressing the NucLight Red fluorescent protein restricted to the nucleus to allow separation from unlabeled human FAP positive NIH/3T3-huFAP clone 19 cells.

    Article Title: Bispecific 2+1 contorsbodies
    Article Snippet: .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. ..

    Article Title: Tumor-targeted agonistic CD28 antigen binding molecules
    Article Snippet: .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. .. The binding to human CD28 was tested with CHO cells expressing human CD28 (parental cell line CHO-kl ATCC #CCL-61, modified to stably overexpress human CD28).

    Article Title: Combination therapy with targeted 4-1BB (CD137) agonists/anti-FAP binding domain and anti-CEA/anti-CD3 bispecific antibody
    Article Snippet: NLV-peptide-specific CD8+ T cells were isolated by FACS sorting using an ARIA cell sorter (BD Bioscience with DIVA software) with the following settings: 100 μm nozzle and purity sort mask. .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP. .. Cells were cultured in DMEM (GIBCO, Cat. No. 42430-082) containing 10% calf serum (Sigma-Aldrich, Cat. No. C8056-500 ml, gamma-irradiated, mycoplasma-free and heat inactivated at 56° C. for 35 min) and 1.5 μg/mL Puromycin (Sigma-Aldrich, Cat. No. ant-pr-1). b) Preparation of Effector cells Buffy coats were obtained from the Zürich blood donation center.

    Article Title: Bispecific antigen binding molecules capable of specific binding to CD40 and to fap
    Article Snippet: .. NIH/3T3-huFAP clone 19 and NIH/3T3-mFAP clone 26 were generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express under 1.5 μg/mL Puromycin selection huFAP or mFAP, respectively. ..

    Article Title: Methods of treating cancer by administering tumor necrosis factor (TNF) family ligand trimer-containing antigen-binding molecules
    Article Snippet: .. NIH/3T3-huFAP clone 39 was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. .. The surface expression of FAP was quantified using the Quifikit (Dako Cat. No. K0078) according to manufactures instructions.

    Article Title: Bispecific antibodies specific for OX40
    Article Snippet: 4.3.2.2 Binding to Human FAP-Expressing Tumor Cells The binding to cell surface FAP was tested using human fibroblast activating protein (huFAP) expressing cells NIH/3T3-huFAP clone 39 or WM266-4 cells (ATCC CRL-1676). .. NIH/3T3-huFAP clone 39 was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. .. In some assays WM266-4 cells were pre-labeled with PKH-26 Red Fluorescence Cell linker Kit (Sigma, Cat.-No. PKH26GL) as described in Example 2.3.2 to allow separation of these tumor cells from other cells present (e.g. human PBMC).

    Expressing:

    Article Title: Bispecific antigen binding molecules targeting OX40 and FAP
    Article Snippet: .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. .. The lack of binding to OX40 negative FAP negative tumor cells was tested using A549 NucLightTM Red Cells (Essen Bioscience, Cat. No. 4491) expressing the NucLight Red fluorescent protein restricted to the nucleus to allow separation from unlabeled human FAP positive NIH/3T3-huFAP clone 19 cells.

    Article Title: Bispecific 2+1 contorsbodies
    Article Snippet: .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. ..

    Article Title: Tumor-targeted agonistic CD28 antigen binding molecules
    Article Snippet: .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. .. The binding to human CD28 was tested with CHO cells expressing human CD28 (parental cell line CHO-kl ATCC #CCL-61, modified to stably overexpress human CD28).

    Article Title: Combination therapy with targeted 4-1BB (CD137) agonists/anti-FAP binding domain and anti-CEA/anti-CD3 bispecific antibody
    Article Snippet: NLV-peptide-specific CD8+ T cells were isolated by FACS sorting using an ARIA cell sorter (BD Bioscience with DIVA software) with the following settings: 100 μm nozzle and purity sort mask. .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP. .. Cells were cultured in DMEM (GIBCO, Cat. No. 42430-082) containing 10% calf serum (Sigma-Aldrich, Cat. No. C8056-500 ml, gamma-irradiated, mycoplasma-free and heat inactivated at 56° C. for 35 min) and 1.5 μg/mL Puromycin (Sigma-Aldrich, Cat. No. ant-pr-1). b) Preparation of Effector cells Buffy coats were obtained from the Zürich blood donation center.

    Article Title: Bispecific antigen binding molecules capable of specific binding to CD40 and to fap
    Article Snippet: .. NIH/3T3-huFAP clone 19 and NIH/3T3-mFAP clone 26 were generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express under 1.5 μg/mL Puromycin selection huFAP or mFAP, respectively. ..

    Article Title: Methods of treating cancer by administering tumor necrosis factor (TNF) family ligand trimer-containing antigen-binding molecules
    Article Snippet: .. NIH/3T3-huFAP clone 39 was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. .. The surface expression of FAP was quantified using the Quifikit (Dako Cat. No. K0078) according to manufactures instructions.

    Article Title: Bispecific antibodies specific for OX40
    Article Snippet: 4.3.2.2 Binding to Human FAP-Expressing Tumor Cells The binding to cell surface FAP was tested using human fibroblast activating protein (huFAP) expressing cells NIH/3T3-huFAP clone 39 or WM266-4 cells (ATCC CRL-1676). .. NIH/3T3-huFAP clone 39 was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. .. In some assays WM266-4 cells were pre-labeled with PKH-26 Red Fluorescence Cell linker Kit (Sigma, Cat.-No. PKH26GL) as described in Example 2.3.2 to allow separation of these tumor cells from other cells present (e.g. human PBMC).

    Selection:

    Article Title: Bispecific antigen binding molecules targeting OX40 and FAP
    Article Snippet: .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. .. The lack of binding to OX40 negative FAP negative tumor cells was tested using A549 NucLightTM Red Cells (Essen Bioscience, Cat. No. 4491) expressing the NucLight Red fluorescent protein restricted to the nucleus to allow separation from unlabeled human FAP positive NIH/3T3-huFAP clone 19 cells.

    Article Title: Bispecific 2+1 contorsbodies
    Article Snippet: .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. ..

    Article Title: Tumor-targeted agonistic CD28 antigen binding molecules
    Article Snippet: .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. .. The binding to human CD28 was tested with CHO cells expressing human CD28 (parental cell line CHO-kl ATCC #CCL-61, modified to stably overexpress human CD28).

    Article Title: Bispecific antigen binding molecules capable of specific binding to CD40 and to fap
    Article Snippet: .. NIH/3T3-huFAP clone 19 and NIH/3T3-mFAP clone 26 were generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express under 1.5 μg/mL Puromycin selection huFAP or mFAP, respectively. ..

    Article Title: Methods of treating cancer by administering tumor necrosis factor (TNF) family ligand trimer-containing antigen-binding molecules
    Article Snippet: .. NIH/3T3-huFAP clone 39 was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. .. The surface expression of FAP was quantified using the Quifikit (Dako Cat. No. K0078) according to manufactures instructions.

    Article Title: Bispecific antibodies specific for OX40
    Article Snippet: 4.3.2.2 Binding to Human FAP-Expressing Tumor Cells The binding to cell surface FAP was tested using human fibroblast activating protein (huFAP) expressing cells NIH/3T3-huFAP clone 39 or WM266-4 cells (ATCC CRL-1676). .. NIH/3T3-huFAP clone 39 was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. .. In some assays WM266-4 cells were pre-labeled with PKH-26 Red Fluorescence Cell linker Kit (Sigma, Cat.-No. PKH26GL) as described in Example 2.3.2 to allow separation of these tumor cells from other cells present (e.g. human PBMC).

    Plasmid Preparation:

    Article Title: Bispecific antigen binding molecules targeting OX40 and FAP
    Article Snippet: .. This cell line was generated by the transfection of the mouse embryonic fibroblast NIH/3T3 cell line (ATCC CRL-1658) with the expression vector pETR4921 to express huFAP under 1.5 μg/mL Puromycin selection. ..



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    Representative image of the subcutaneous implantation site of the NIH/3T3 group. H&E shows a large subcutaneous mass and ink in the middle. The upper left corner shows a detail of the ink-bearing cells. The picture was taken at 10×; 80 µm. Close-up picture (upper left corner) was taken at 40×; 20 µm.

    Journal: Cells

    Article Title: Preclinical Tumorigenicity Study of an Advanced Therapy Medicinal Product for Diffuse Cartilage Lesions in an Osteoarthritic Environment

    doi: 10.3390/cells15050429

    Figure Lengend Snippet: Representative image of the subcutaneous implantation site of the NIH/3T3 group. H&E shows a large subcutaneous mass and ink in the middle. The upper left corner shows a detail of the ink-bearing cells. The picture was taken at 10×; 80 µm. Close-up picture (upper left corner) was taken at 40×; 20 µm.

    Article Snippet: The mouse embryonic fibroblast cell line NIH/3T3 (ATCC; LGC Standards GmbH, Wesel, Germany) was used as a negative control in this study.

    Techniques: